Catálogo de publicaciones - revistas

<jats:p> Li <jats:italic>et al</jats:italic> . (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported large numbers of differences between DNA and messenger RNA in human cells, indicating unprecedented levels of RNA editing, and including sequence changes not produced by any of the known RNA editing mechanisms. However, common sources of systematic errors in high-throughput sequencing technology, which were not properly accounted for in this study, explain most of the claimed differences. </jats:p>

<jats:p> Li <jats:italic>et al</jats:italic> . <jats:bold>(</jats:bold> Research Articles, 1 July 2011, p. 53; published online 19 May 2011 <jats:bold>)</jats:bold> reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these sequence mismatches can likely be explained by technical artifacts such as errors in mapping sequencing reads to a reference genome, sequencing errors, and genetic variation. </jats:p>

<jats:p> Li <jats:italic>et al</jats:italic> . (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported widespread differences between the RNA and DNA sequences of the same human cells, including all 12 possible mismatch types. Before accepting such a fundamental claim, a deeper analysis of the sequencing data is required to discern true differences between RNA and DNA from potential artifacts. </jats:p>