Catálogo de publicaciones - revistas

<jats:title>Multiplexed RNA imaging in single cells</jats:title> <jats:p> The basis of cellular function is where and when proteins are expressed and in what quantities. Single-molecule fluorescence in situ hybridization (smFISH) experiments quantify the copy number and location of mRNA molecules; however, the numbers of RNA species that can be simultaneously measured by smFISH has been limited. Using combinatorial labeling with error-robust encoding schemes, Chen <jats:italic>et al.</jats:italic> simultaneously imaged 100 to 1000 RNA species in a single cell. Such large-scale detection allows regulatory interactions to be analyzed at the transcriptome scale. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" related-article-type="in-this-issue" xlink:href="10.1126/science.aaa6090">10.1126/science.aaa6090</jats:related-article> </jats:p>

<jats:p>Big science is hard. It is the throw-deep approach that pushes technology to the edge to achieve stunning breakthroughs that dramatically extend the frontiers of science, while inspiring the next generation of scientists and capturing the imagination of the public. The Large Hadron Collider (LHC) produced the Higgs boson, a particle that explains why all other particles have mass and whose name is now known around the world; the Atacama Large Millimeter and Submillimeter Array (ALMA) revealed some of the earliest galaxies, as well as a nearby planetary system that has a striking resemblance to our own; and for 25 years, the Hubble Space Telescope has dazzled us with its discoveries and iconic astronomical images. But before they became famous for discoveries, these and other big projects were infamous for their problems. What are the challenges for big science, and what does it take to succeed?</jats:p>

<jats:p>A roundup of weekly science policy and related news.</jats:p>

<jats:p>FDA warning to UCLA highlights struggle to diagnose chronic traumatic encephalopathy.</jats:p>

<jats:p>European Parliament vows to shield science budget from stimulus package raid.</jats:p>

<jats:p>Authorization bill would cut climate and energy research and reshape science agency policies.</jats:p>

<jats:p>Many clinical trials don't help doctors make decisions. A new breed of studies aims to change that.</jats:p>

<jats:p>But other wolves adopt a new Lake Superior island.</jats:p>

<jats:p>Analyses show better scored proposals produce more papers and citations.</jats:p>