Catálogo de publicaciones - revistas

<jats:title>Membrane protein oligomers by design</jats:title> <jats:p> In recent years, soluble protein design has achieved successes such as artificial enzymes and large protein cages. Membrane proteins present a considerable design challenge, but here too there have been advances, including the design of a zinc-transporting tetramer. Lu <jats:italic>et al.</jats:italic> report the design of stable transmembrane monomers, homodimers, trimers, and tetramers with up to eight membrane-spanning regions in an oligomer. The designed proteins adopted the target oligomerization state and localized to the predicted cellular membranes, and crystal structures of the designed dimer and tetramer reflected the design models. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6379" page="1042" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aaq1739">1042</jats:related-article> </jats:p>

<jats:title>The proton channel behind sour taste</jats:title> <jats:p> Although many proteins that form ion channels in cell membranes have been described, none that selectively conduct protons into eukaryotic cells have been identified. Tu <jats:italic>et al.</jats:italic> used a genetic screen to pinpoint candidate genes that might encode such a protein from mouse taste receptor cells (see the Perspective by Montell). They identified the known protein otopetrin and showed that it conferred proton conductance when expressed in cultured human cells. Their results indicate that otopetrin may function in sensory recognition of sour (acidic) taste in humans and other organisms. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6379" page="1047" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aao3264">1047</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6379" page="991" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aas9772">991</jats:related-article> </jats:p>

<jats:title>Tracking regulatory DNA in action</jats:title> <jats:p> Cis-regulatory DNA elements such as enhancers and promoters are critical for transcription regulation. Little is known about the relationship between these elements' transcriptional activity and their mobility within the nucleus in living cells. Gu <jats:italic>et al.</jats:italic> developed a strategy to deliver multiple RNAs to guide inactive Cas9 to label these elements. Quantitative measurement of their movement during stem cell differentiation revealed that increased DNA loci mobility correlated with transcriptional activation. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6379" page="1050" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aao3136">1050</jats:related-article> </jats:p>

<jats:title>An off switch for helminth immunity</jats:title> <jats:p> Group 2 innate lymphoid cells (ILC2s) are involved in responses to helminths, viruses, and allergens. Moriyama <jats:italic>et al.</jats:italic> found that ILC2s interact with the nervous system to modulate helminth immunity. ILC2s from the small intestine expressed the β <jats:sub>2</jats:sub> -adrenergic receptor (β <jats:sub>2</jats:sub> AR), which normally interacts with the neurotransmitter epinephrine. Inactivating β <jats:sub>2</jats:sub> AR resulted in lower helminth burden and more ILC2s, eosinophils, and type 2 cytokine production in mice. Conversely, treatment of helminth-infected mice with a β <jats:sub>2</jats:sub> AR agonist enhanced worm burden and reduced proliferation of ILC2s. Thus, β <jats:sub>2</jats:sub> AR negatively regulates ILC2-driven protective immunity. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6379" page="1056" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aan4829">1056</jats:related-article> </jats:p>

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>