Catálogo de publicaciones - revistas

<jats:title>Taking the heat together</jats:title> <jats:p> Many of the processes in living cells are mediated by protein complexes that dynamically assemble and dissociate depending on cellular needs. Tan <jats:italic>et al.</jats:italic> developed a method called thermal proximity coaggregation (TPCA) to monitor the dynamics of native protein complexes inside cells (see the Perspective by Li <jats:italic>et al.</jats:italic> ). The method is based on the idea that proteins within a complex will coaggregate upon heat denaturation. It uses a previously described cellular shift assay to determine melting curves for thousands of proteins and assigns a TPCA signature on the basis of similarity between the curves. The method was validated by detection of many known protein complexes. It identified cell-specific interactions in six cell lines, highlighting the potential for identifying protein complexes that are modulated by disease. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6380" page="1170" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aan0346">1170</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6380" page="1105" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aat0576">1105</jats:related-article> </jats:p>

<jats:title>Committing the heart</jats:title> <jats:p> The heart is a complex organ composed of multiple cell types such as cardiomyocytes and endothelial cells. Cardiovascular cells arise from <jats:italic>Mesp1</jats:italic> -expressing progenitor cells. Lescroart <jats:italic>et al.</jats:italic> performed single-cell RNA-sequencing analysis of mouse wild-type and <jats:italic>Mesp1</jats:italic> -deficient cardiovascular progenitor cells at early gastrulation (see the Perspective by Kelly and Sperling). When <jats:italic>Mesp1</jats:italic> was eliminated, embryonic cells remained pluripotent and could not differentiate into cardiovascular progenitors. During gastrulation, the different <jats:italic>Mesp1</jats:italic> progenitors rapidly became committed to a particular cell fate and heart region. <jats:italic>Notch1</jats:italic> expression marked the earliest step of cardiovascular lineage segregation. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6380" page="1177" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aao4174">1177</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6380" page="1098" related-article-type="in-this-issue" vol="359" xlink:href="10.1126/science.aat0230">1098</jats:related-article> </jats:p>

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>