Catálogo de publicaciones - revistas

<jats:title>Lipid droplets help anti-TB drug efficacy</jats:title> <jats:p> Improving chemotherapies against intracellular pathogens requires understanding how antibiotic distribution within infected cells affects efficacy. Greenwood <jats:italic>et al.</jats:italic> developed an approach to visualize antibiotics in human macrophages infected with the tubercle bacillus (see the Perspective by Smith and Aldridge). They showed that the antitubercular (anti-TB) drug bedaquiline accumulated in host lipid droplets. Lipid droplets seemed to act as an antibiotic reservoir that could be transferred to bacteria during host lipid consumption. Indeed, alterations in host lipid droplet content affected the anti-TB activity of bedaquiline against intracellular bacilli. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6447" page="1279" related-article-type="in-this-issue" vol="364" xlink:href="10.1126/science.aat9689">1279</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6447" page="1234" related-article-type="in-this-issue" vol="364" xlink:href="10.1126/science.aay0211">1234</jats:related-article> </jats:p>

<jats:title>Positive reinforcement in a GPCR</jats:title> <jats:p> Many drug discovery efforts focus on G protein–coupled receptors (GPCRs), a class of receptors that regulate many physiological processes. An exemplar is the β <jats:sub>2</jats:sub> -adrenergic receptor (β <jats:sub>2</jats:sub> AR), which is targeted by both blockers and agonists to treat cardiovascular and respiratory diseases. Most GPCR drugs target the primary (orthosteric) ligand binding site, but binding at allosteric sites can modulate activation. Because such allosteric sites are less conserved, they could possibly be targeted more specifically. Liu <jats:italic>et al.</jats:italic> report the crystal structure of β <jats:sub>2</jats:sub> AR bound to both an orthosteric agonist and a positive allosteric modulator that increases receptor activity. The structure suggests why the modulator compound is selective for β <jats:sub>2</jats:sub> AR over the closely related β <jats:sub>1</jats:sub> AR. Furthermore, the structure reveals that the modulator acts by enhancing orthosteric agonist binding and stabilizing the active conformation of the receptor. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6447" page="1283" related-article-type="in-this-issue" vol="364" xlink:href="10.1126/science.aaw8981">1283</jats:related-article> </jats:p>

<jats:title>Variants affect gene expression over time</jats:title> <jats:p> Genetic variation drives the spectrum of human phenotypes but in some cases has been linked to pathological conditions. Strober <jats:italic>et al.</jats:italic> set out to explore how genetic diversity regulates gene expression in cell differentiation over time. They examined expression quantitative trait loci (eQTLS)—genetic variants correlated with gene expression—in induced pluripotent stem cell lines from 19 Yoruban individuals at 16 time points during differentiation into cardiomyocytes. They identified hundreds of developmental stage–specific eQTLs. Thus, the impact of genetic variations depends on the developmental context or tissue under observation. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6447" page="1287" related-article-type="in-this-issue" vol="364" xlink:href="10.1126/science.aaw0040">1287</jats:related-article> </jats:p>

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>