Catálogo de publicaciones - revistas

<jats:title>Expanding the human proteome</jats:title> <jats:p> Using mass spectrometry, ribosome profiling, and several CRISPR-based screens, Chen <jats:italic>et al.</jats:italic> identified hundreds of previously uncharacterized functional micropeptides in the human genome (see the Perspective by Wei and Guo). Protein translation outside of annotated open reading frames (ORFs) in messenger RNAs and within ORFs in long noncoding RNAs is pervasive. A functional screen using CRISPR-Cas9 with single-cell transcriptomics suggested critical roles for hundreds of micropeptides. Micropeptides encoded by multiple short, upstream ORFs form stable protein complexes with the downstream canonical proteins encoded on the same messenger RNAs. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6482" page="1140" related-article-type="in-this-issue" vol="367" xlink:href="10.1126/science.aay0262">1140</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6482" page="1074" related-article-type="in-this-issue" vol="367" xlink:href="10.1126/science.aba6117">1074</jats:related-article> </jats:p>

<jats:title>Porin' through the wax</jats:title> <jats:p> <jats:italic>Mycobacterium tuberculosis</jats:italic> has a distinctive physiology that allows it to persist in the human body, including a wax-like cell coat that is largely impermeable and can resist attack by host immune response effectors. Wang <jats:italic>et al.</jats:italic> identified a simple molecule that effectively crosses this cell coat and kills <jats:italic>M. tuberculosis</jats:italic> cells. Whole-genome sequencing of mutants resistant to this molecule, 3,3- <jats:italic>bis</jats:italic> -di(methylsulfonyl)propionamide, showed scattered mutations in a protein called PPE51, and these mutants resulted in a spectrum of nutrient-related growth defects. Experiments suggest that PE/PPE family proteins are small molecule–selective channels analogous to outer membrane porins, which allow <jats:italic>M. tuberculosis</jats:italic> to take up nutrients while maintaining an otherwise impermeable barrier. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6482" page="1147" related-article-type="in-this-issue" vol="367" xlink:href="10.1126/science.aav5912">1147</jats:related-article> </jats:p>

<jats:title>RNA life span at single-cell resolution</jats:title> <jats:p> RNA transcripts are an easily accessed representation of gene expression, but we lack a comprehensive view of the life span of RNA within the single cell. Battich <jats:italic>et al.</jats:italic> developed a method to sequence messenger RNA labeled with 5-ethynyl-uridine (EU) in single cells (scEU-seq), which allows estimation of RNA transcription and degradation rates. When examining intestinal organoid cells, scEU-seq data can be used to discern between transcription and degradation during development, indicating that this method can be applied to better understand the relationship between gene expression and RNA degradation during development. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6482" page="1151" related-article-type="in-this-issue" vol="367" xlink:href="10.1126/science.aax3072">1151</jats:related-article> </jats:p>

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