Catálogo de publicaciones - tesis
Título de Acceso Abierto
Estudio de la capacidad antigénica e inmunogénica de péptidos sintéticos correspondientes a epitopes conservados de las proteínas estructurales del virus de la anemia infecciosa equina
Adriana Rosa Soutullo Ileana Malan Borel Eduardo Gimeno Ana María Sadir Élida Álvarez Georgina Tonarelli
acceptedVersion.
Resumen/Descripción – provisto por el repositorio digital
Equine Infectious Anemia (EIA) is an infectious disease caused by a lentivirus, a life-long persistent and asymptomatic infection. The virus has three principal structural proteins, gp90, gp45 and p26, capable of inducing an effector immune responses during this stage to eliminate the ancestral viral quasispecies but no epitopes T and/or B have been identified to induce it yet. Based on the above statements, in this work we analyze the antigenic and immunogenic capacity of three synthetic peptides gp90(416-444), gp45(522-546), and p26(318-346), which mimic conserved regios of the principal structural proteins of the EIA virus. Antigenicity was evaluated through ELISA and lymphoproliferation in sera and lymphocytes from persistently infected horses and immunogenicity through study the humoral and cellular immune response in BALB/c mice, inoculated with 0, 20 and 100 μg of each peptide /animal. The reactivity of equine sera and lymphocytes from infected animals showed that gp90(416-444), gp45(522-546) have been recognized more than p26(318-346). In mice inoculated it was shown, that gp90(416-444), and gp45(522-546) were capable of producing greater antibody synthesis than p26(318-346). Indeed, the evaluation of the cellular immune response showed that gp90(416-444) was capable of stimulating the production of IFNgamma, whereas gp45(522-546) and p26(318-346) stimulated proliferation of lymphocytes from animals inoculated with these peptides. Upon these results, we can concluded that the peptides gp90(416-444) and gp45(522-546), could be included in a vaccine to protect horses from EIAV.Palabras clave – provistas por el repositorio digital
Equine infectious anemia; Synthetic peptides; Gp90; Gp45; P26; Antigenicity; Immunogenicity; Anemia infecciosa equina; Péptidos sintéticos; Antigenicidad; Inmunogenicidad
Disponibilidad
| Institución detectada | Año de publicación | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No requiere | 2008 | Biblioteca Virtual de la Universidad Nacional del Litoral (SNRD) |
|
Información
Tipo de recurso:
tesis
Idiomas de la publicación
- español castellano
País de edición
Argentina
Fecha de publicación
2008-12-10