Catálogo de publicaciones - revistas

<jats:title>Patterns of development regulation within tissues</jats:title> <jats:p> Expression of a given gene at the RNA level does not always correlate with expression at the protein level for many organisms. Walley <jats:italic>et al.</jats:italic> have built an integrated atlas of gene expression and regulatory networks in developing maize, using the same tissue samples to measure the transcriptome, proteome, and phosphoproteome. Coexpression networks from the transcriptome and proteome showed little overlap with each other, even though they showed enrichment of similar pathways. Integration of mRNA, protein, and phosphoprotein data sets improved the predictive power of the gene regulatory networks. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6301" page="814" related-article-type="in-this-issue" vol="353" xlink:href="10.1126/science.aag1125">814</jats:related-article> </jats:p>

<jats:title>Recoding and repurposing genetic codons</jats:title> <jats:p> By recoding bacterial genomes, it is possible to create organisms that can potentially synthesize products not commonly found in nature. By systematic replacement of seven codons with synonymous alternatives for all protein-coding genes, Ostrov <jats:italic>et al.</jats:italic> recoded the <jats:italic>Escherichia coli</jats:italic> genome. The number of codons in the <jats:italic>E. coli</jats:italic> genetic code was reduced from 64 to 57 by removing instances of the UAG stop codon and excising two arginine codons, two leucine codons, and two serine codons. Over 90% functionality was successfully retained. In 10 cases, reconstructed bacteria were not viable, but these few failures offered interesting insights into genome-design challenges and what is needed for a viable genome. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6301" page="819" related-article-type="in-this-issue" vol="353" xlink:href="10.1126/science.aaf3639">819</jats:related-article> </jats:p>

<jats:title>Characterizing the Zika virus antibody response</jats:title> <jats:p> Given the public health emergency that Zika virus poses, scientists are seeking to understand the Zika-specific immune response. Stettler <jats:italic>et al.</jats:italic> analyzed 119 monoclonal antibodies isolated from four donors that were infected with Zika virus during the present epidemic, including two individuals that had previously been infected with dengue virus, another member of the flavivirus family. Neutralizing antibodies primarily recognized the envelope protein domain III (EDIII) or quaternary epitopes on the intact virus, and an EDIII-targeted antibody protected mice against lethal infection. Some EDI/II-targeting antibodies cross-reacted with dengue virus in vitro and could enhance disease in dengue-infected mice. Whether dengue and Zika virus antibodies cross-react in humans remains to be tested. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6301" page="823" related-article-type="in-this-issue" vol="353" xlink:href="10.1126/science.aaf8505">823</jats:related-article> </jats:p>

<jats:title>Genetic variation and coronary artery disease</jats:title> <jats:p> Most genetic variants lie outside protein-coding genes, but their effects, especially in human health, are not well understood. Franzén <jats:italic>et al.</jats:italic> examined gene expression in tissues affected by coronary artery disease (CAD). They found that individuals with loci that have been associated with CAD in genome-wide analyses had different patterns of tissue-specific gene expression than individuals without these genetic variants. Similarly, tissues not associated with CAD did not have CAD-like expression patterns. Thus, tissue-specific data can be used to dissect the genetic effects that predispose individuals to CAD. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6301" page="827" related-article-type="in-this-issue" vol="353" xlink:href="10.1126/science.aad6970">827</jats:related-article> </jats:p>

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>