Catálogo de publicaciones - revistas

<jats:title>Bringing carbon-silicon bonds to life</jats:title> <jats:p> Organic compounds containing silicon are important for a number of applications, from polymers to semiconductors. The catalysts used for creating carbon-silicon bonds, however, often require expensive trace metals or have limited lifetimes. Borrowing from the ability of some metallo-enzymes to catalyze other rare carbene insertion reactions, Kan <jats:italic>et al.</jats:italic> used heme proteins to form carbon-silicon bonds across a range of conditions and substrates (see the Perspective by Klare and Oestreich). Directed evolution experiments using cytochrome c from <jats:italic>Rhodothermus marinus</jats:italic> improved the reaction to be 15 times more efficient than industrial catalysts. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6315" page="1048" related-article-type="in-this-issue" vol="354" xlink:href="10.1126/science.aah6219">1048</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6315" page="970" related-article-type="in-this-issue" vol="354" xlink:href="10.1126/science.aal1951">970</jats:related-article> </jats:p>

<jats:p>The diffraction limit for light microscopy (approximately half the wavelength of light used to image a sample) was for many years a barrier to truly high-resolution microscopy. Superresolution microscopy came about through the recognition that clever hardware design and digital image processing could break through the diffraction limit, enabling scientists to image down to the molecular level. Of all the superresolution techniques, structured illumination microscopy (SIM) is probably the most relevant for live samples, primarily because it is the most versatile and suitable for fairly long exposures and dynamic cell imaging. Stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM), as well as techniques derived from them, provide higher-resolution images but tend to require considerably more laser power than SIM—making phototoxicity a significant problem. This webinar will provide a summary of superresolution microscopy, with a particular focus on SIM, and will highlight the important factors to consider when undertaking microscopy experiments, including hardware, software, and samples.</jats:p>

<jats:p>Understanding how a particular DNA sequence gives rise to a particular protein provides us with some insight into that protein, but a deeper investigation of how the protein is made, where it is located, and how much of it is present in different cell types is required to enable a true understanding of its function. Proteomics researchers seek to identify and characterize each and every protein in a specific location—be it the whole body or a specific tissue. This poster aims to delve down an additional layer into the cellular proteome, taking a journey into the subcellular compartments within a single cell to discover what secrets each organellar proteome might hold. Characterizing the distribution of individual proteins at a subcellular level provides important clues to the pathways and processes in which these proteins are involved.</jats:p> <jats:p> This special supplement brought to you by the <jats:bold>Science</jats:bold> /AAAS Custom Publishing Office. </jats:p>

<jats:p>Cell biologists no longer rely only on pictures from microscopes—pixels and pulses from cell sorters and other devices now enhance their research. By borrowing tools and techniques from computer scientists and engineers, they have upgraded their approach to studying cell morphology.</jats:p>

<jats:p>Understanding how a particular DNA sequence gives rise to a particular protein provides us with some insight into that protein, but a deeper investigation of how the protein is made, where it is located, and how much of it is present in different cell types is required to enable a true understanding of its function. Proteomics researchers seek to identify and characterize each and every protein in a specific location—be it the whole body or a specific tissue. This poster aims to delve down an additional layer into the cellular proteome, taking a journey into the subcellular compartments within a single cell to discover what secrets each organellar proteome might hold. Characterizing the distribution of individual proteins at a subcellular level provides important clues to the pathways and processes in which these proteins are involved.</jats:p> <jats:p> This special supplement brought to you by the <jats:bold>Science</jats:bold> /AAAS Custom Publishing Office. </jats:p>

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>