Catálogo de publicaciones - revistas

<jats:title>Breaking down miRNAs</jats:title> <jats:p> Although much work has examined microRNA (miRNA) biogenesis, relatively little is known about miRNA decay. Elbarbary <jats:italic>et al.</jats:italic> now identify Tudor-SN, an endonuclease that interacts with the RNA-induced silencing complex. Tudor-SN targets miRNAs at CA and UA dinucleotides located more than five nucleotides from miRNA ends. Tudor-SN-mediated miRNA decay removes miRNAs that silence genes encoding proteins that are critical for the G <jats:sub>1</jats:sub> -to-S phase transition in the cell cycle. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6340" page="859" related-article-type="in-this-issue" vol="356" xlink:href="10.1126/science.aai9372">859</jats:related-article> </jats:p>

<jats:title>Trapping RNA polymerase in the act</jats:title> <jats:p> The enzyme RNA polymerase (RNAP) finds promoter elements in the genome, separates (or “melts”) the DNA strands, and transcribes the template DNA strand to give RNA. A mobile clamp in RNAP plays a key role in initiating transcription. Feklistov <jats:italic>et al.</jats:italic> locked the clamp of bacterial RNAP in distinct conformations by using small molecules. They then used fluorescent probes to monitor binding as the promoter DNA was separated. Unexpectedly, they found that the clamp transiently closed to nucleate DNA melting, opened to load single-stranded DNA into the active site, and then closed around the template strand to start transcription. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6340" page="863" related-article-type="in-this-issue" vol="356" xlink:href="10.1126/science.aam7858">863</jats:related-article> </jats:p>

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>