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Institución detectada	Período	Navegá	Descargá	Solicitá
No detectada	desde mar. 1997 / hasta dic. 2023	Science Journals

Información

Tipo de recurso:

revistas

ISSN impreso

0036-8075

ISSN electrónico

1095-9203

Editor responsable

American Association for the Advancement of Science (AAAS)

País de edición

Estados Unidos

Fecha de publicación

1880-

Cobertura temática

Ciencias naturales

Tabla de contenidos

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doi: 10.1126/science.aat7992

Semisynthetic sensor proteins enable metabolic assays at the point of care

Qiuliyang Yu; Lin Xue; Julien Hiblot; Rudolf Griss; Sebastian Fabritz; Clothilde Roux; Pierre-Alain Binz; Dorothea Haas; Jürgen G. Okun; Kai Johnsson

<jats:title>A protein designed to sense metabolites</jats:title> <jats:p> Many diseases cause characteristic changes in blood metabolites. Yu <jats:italic>et al.</jats:italic> describe a paper-based assay in which a chosen metabolite can be oxidized to generate reduced nicotinamide adenine dinucleotide phosphate (NADPH). Color changes in a designed NADPH sensor protein are then quantified by a digital camera. The sensor system successfully generated point-of-care measurements of phenylalanine, glucose, and glutamate. Concentrations of phenylalanine in the blood of phenylketonuria patients were analyzed within minutes with only half a microliter of blood. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6407" page="1122" related-article-type="in-this-issue" vol="361" xlink:href="10.1126/science.aat7992">1122</jats:related-article> </jats:p>

Palabras clave: Multidisciplinary.

Pp. 1122-1126

doi: 10.1126/science.aat2477

Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining

John M. Pryor; Michael P. Conlin; Juan Carvajal-Garcia; Megan E. Luedeman; Adam J. Luthman; George W. Small; Dale A. Ramsden

<jats:title>RNA takes over DNA repair</jats:title> <jats:p> Damage to DNA genomes is normally thought to be repaired with DNA. Pryor <jats:italic>et al.</jats:italic> now describe a clear exception (see the Perspective by Modesti). They found that RNA is routinely incorporated during the repair of DNA double-strand breaks through the mammalian nonhomologous end–joining (NHEJ) pathway. In a variety of contexts, including V(D)J recombination and Cas9-induced genome engineering, two “DNA” polymerases specific to NHEJ preferentially added RNA in cells. These RNA additions facilitated the critical step of ligation and were later replaced by DNA to complete the NHEJ repair process. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6407" page="1126" related-article-type="in-this-issue" vol="361" xlink:href="10.1126/science.aat2477">1126</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6407" page="1069" related-article-type="in-this-issue" vol="361" xlink:href="10.1126/science.aau9194">1069</jats:related-article> </jats:p>

Palabras clave: Multidisciplinary.

Pp. 1126-1129

doi: 10.1126/science.361.6407.1130

New Products

<jats:p>A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.</jats:p>

Palabras clave: Multidisciplinary.

Pp. 1130-1130

doi: 10.1126/science.361.6407.1158